A plasmid contains a multiple cloning site inside the coding region of the lacZgene and also contains an ampicillin resistance gene at a separate locus. When cells are transformed with a successfully recombinant plasmid containing a piece of DNA cloned into the multiple cloning site, what color will the colonies be when grown on an agar plate containing ampicillin and X-gal

Answers

Answer 1

Answer:

The correct option is white.

Explanation:

As the options are not given, the options are found online and are as follows;

(a.) green (b.) white (c.) colonies will not grow (d.) blue (e.) clear

Out of this, white is the true option because in recombinant plasmid lac Z gene is disturbed and cannot produce beta galactosidase enzyme and there is no hydrolysis of x gal and no blue so recombinant plasmid gives white colonies)


Related Questions

4) What factors influence the circulation of the Earth's atmosphere,
thereby producing our weather?

Answers

The movement of air through Earth's -- or any planet's -- atmosphere is called wind, and the main cause of Earth's winds is uneven heating by the sun. This uneven heating causes changes of atmospheric pressure, and winds blow from regions with high pressure to those with low pressure

The uneven heating of the earth's surface is one of the main variables affecting the circulation of the atmosphere globally along with  seasonal variations in precipitation and temperature and Earth turning on its axis.

What is weather?

The state of the atmosphere is referred to as the weather, and can be described in a number of different ways, including the degree to which it is hot or cold, wet or dry, quiet or stormy, clear or cloudy. The troposphere, the lowest layer of the Earth's atmosphere, is where the majority of meteorological events take place. The stratosphere is the next layer up from the troposphere. The term "weather" is used to describe the temperature, precipitation, and other characteristics of the atmosphere on a day-to-day basis.

Variations in air pressure, temperature, and moisture from one location to another are the primary contributors to a day's weather. These disparities can be attributed to the fact that the angle at which the Sun is positioned at any given location varies with latitude. The vast temperature difference between the polar and tropical air masses is what gives rise to the biggest scale atmospheric circulations.

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Which type of electromagnetic wave is the most similar in wavelength to visible light?

A. Radio Waves
B. Gamma radiation
C. Ultraviolet light (UV radiation)
D. Sound waves

Answers

Answer:

uv

Explanation:

im pretty sure

State and discuss two types of immunity.

Answers

Answer:

How Does the Immune System Work?

Innate immunity: Everyone is born with innate (or natural) immunity, a type of general protection. ...

Adaptive immunity: Adaptive (or active) immunity develops throughout our lives. ...

Passive immunity: Passive immunity is "borrowed" from another source and it lasts for a short time.

5.Most of the fish in a lake produce a certain number of eggs each spring. However, one fish has the ability to carry and lay more fish eggs.

This is a ________ advantage that allows for ___________________.Lector inmersivo
(1 Punto)

survival; the fish to live longer.

reproductive; the fish to produce more offspring.

reproductive; the fish to swim faster.

survival; the fish to produce offspring more often.

Answers

Answer:

reproductive; and produce offspring

brainliest pls

What does it mean to "induce" your cells?​

Answers

Answer:

Induction, in embryology, process by which the presence of one tissue influences the development of others.

What is required for reactants to form bonds?

Answers

Answer:

Energy input is required for the reactants to form bonds.

Grasses → mice → cat → coyote A natural disaster of a fire has occurred what could happen to this food chain? The grasses will be gone and all of the consumers will be able to leave. The grasses will be gone and some of the mice will die but the number of cats and coyotes will remain the same. The grasses will be gone but the number of consumers will all stay the same. The grasses will be gone and any surviving consumers will have to look for new resources in that environment. Nothing will happen to this food chain.

Answers

Answer:

The grasses will be gone and any surviving consumers will have to look for new resources in that environment.

Explanation:

The fire will destroy the grass and harm some of the consumers. Without grass, the mice population will drop and that will cause the cat population to drop and that will lead to the coyote population to also decline. The remaining consumers will have to move to find further resources to survive.

Answer:

The answer will be D

Hope this helps!

Two plants are named Vinca minor and
Vinca rosea. Are they close relatives?
Explain.

Answers

Two plants are named Vinca minor and Vinca rosea. Are they close relatives? Explain. Yes they are close relatives because the share the same genus name and only their species name is different

Where would you expect to find humans with the darkest skin color in the Northern Hemisphere (from 10°N to 60°N)?

Answers

Answer: Australia

Explanation:

Complete the statement about genetic variation.
Meiosis contributes to genetic variability when
the chromosomes line up randomly during
metaphase 1 anaphase I prophase I. This
random line-up means that when the cell divides,
the number of chromosomes in each cell is the
same, but the contents are not. This process is
called | independent assortment |gene linkage |
crossing over|. During meiosis, |gene linkage|
|independent assortment | crossing over | increases
genetic variation because it shuffles portions of
chromosomes around.

Answers

Answer:

1. Metaphase 1

2. Independent assortment

3. Crossing over

Explanation:

Meiosis is a kind of cell division that reduces the chromosome number of the parent cell by half. It only occurs during sexual reproduction and ensures genetic variation in sexually reproducing organisms.

Meiosis contributes to genetic variability when the chromosomes line up randomly at the cell plate during METAPHASE 1 of meiosis. This process of random alignment is called INDEPENDENT ASSORTMENT of genes. Generally, meiosis is able to ensure genetic variability due to a process called CROSSING OVER. Crossing over is a process whereby nonsister chromatids of homologous chromosomes exchanges/shuffles genetic contents.

Please help me name each letter and match it to the definition. (Do not spam on my question either please)

Answers

Answer:

1. B. Medulla oblongata contains cardiac, respiratory, vasomotor and reflex centers.

2. A. Hypothalamus is the major regulator of ANS

3. D. Occipital lobe is the location of visual cortex.

4. F. Sensory areas are primary site responsible for perceiving cutaneous sensory sensations and proprioception.

5. E. Motor areas directs conscious motor movement

6. C. Cerebellum - coordinates movement by comparing intended movement with actual movement.

7. K. Corpus callosum allows communication between right & left cerebral hemispheres.

8. J. Frontal Lobe - Cognition, personality.

9. A. Hypothalamus - Contains hunger, thirst and thermoregulatory centers.

3. What charge does the hydrogen atoms have in a molecule of water?

Answers

Answer: Opposite charges attract one another. The slight positive charges on the hydrogen atoms in a water molecule attract the slight negative charges on the oxygen atoms of other water molecules. This tiny force of attraction is called a hydrogen bond. This bond is very weak.

Answer:

Negative

Very small charge in water.

What are the causes of soil compaction? (Site 1)

Answers

Answer:

Internal factors include particle-size distribution, organic matter content, mineralogy and water content of the soil. Externally applied forces are generally mechanically applied forces, the primary source of these being vehicular traffic

Explanation:

Which influence has immigration and migration had on the population of Texas?

A.) It has kept the growth rate of Texas the same as the growth rate of the United
States.

B.) It lowered the amount of cultural and economic activity in Texas.

C.) It contributed to the 38% of Hispanic Texans as of 2010.

D.) It decreases the need for public services in rural communities.

Answers

I would think it is C but I’m sorry if it’s wrong

Answer:

The answer is choice C.

Explanation:

In this question, I will use the following format: two d*mbs, a trick, & a true. In this strategy, there are two d*mb answers, one answer made to trick the reader, and one true answer (which is the correct answer).

The two d*mbs of this problem are choices B and D. B simply makes no sense since immigration and migration would increase the amount of cultural and economic activity in Texas if anything. I feel as though D is illogical because it is more likely that immigration and migration would increase the need for public services in rural communities. Due to B and D being the opposite of what is necessary for the answers to be true, they can both be swiftly eliminated.

A is the trick of this question. Although it may seem valid at first, there is a fatal flaw about it: the fact that Texas is within the United States. Texas, despite its size and population, is one singular part of the US. It is impossible for Texas to be the only part of the United States to grow. As a result, the growth rate of Texas can never be the same as the growth rate of the United States.

C is the true in this problem. Immigration and migration contributing to the 38% of Hispanic Texans (as of 2010) is indeed a logical answer that adds up. Additionally, when looking at the official resources that this question is derived from, the text clearly states that 'The numbers and percentages of Hispanics in Texas grew greatly between 2000 and 2010. About 2.8 million more Texans identified themselves as Hispanic in 2010 compared to 2000. Hispanics represented 38 percent of all Texans compared to about 32 percent in 2000.' While this information may not seem all that important at a first glance, it actually supports this answer quite a bit. The text clearly provides valid proof of answer choice C; it gives the cause of answer choice C quite plainly and undeniably.

This means that A is the trick, B and D are the d*mbs, and C is the true. Due to the evidence and verification previously stated in my answers, it is quite clear (to me) that the answer is indeed choice C.

In case you're wondering, the words with apostrophes are * d u m b * and * d u m b s * . I get why they censored them, so I simply accommodated and blocked the vowel.

Undersea vents called black smokers release superheated, mineral-rich water. Light does not penetrate the water, making photosynthesis impossible. However, diverse communities of organisms exist around black smokers at depths of 2100 m below the ocean surface.

Which is the most likely reason these diverse communities can exist so deep in the ocean?
Group of answer choices

Black smokers are warm like tropical land areas.

Black smokers produce sulfur that blocks light and creates a good hideaway for many organisms.

Dead organisms that serve as bacterial food sources drift down from water close to the surface.

Producer organisms use minerals in the water to make food through chemosynthesis.

Answers

Answer: Producer organisms use minerals in the water to make food through chemosynthesis.

Explanation:

The producer organisms that exist around these underwater vents are able to produce food through an alternative process to photosynthesis known as Chemosynthesis.

With Chemosynthesis, producer organisms use the minerals in the water from black smokers by converting molecules that contain carbon to organic matter by using energy they acquired from oxidizing ferrous ions and other inorganic compounds.

A tall pea plant results from a dominant tall allele while the short phenotype results from two recessive alleles. What is the frequency of short offspring (pp) when a tall pea plant with one tall allele (Pp) is cross-pollinated with a short pea plant (pp)?

Answers

Answer:

0.5

Explanation:

Took the test xd ♡

The probability of short pea plant when a heterozygous parent cross with recessive is 0.5.

Law of dominance:

The cross between two pure parents results in the heterozygous dominant offspring in the first generation.

Here,

Parents are

P- dominant tall

p - recessive

Pp   x pp

The cross,

        p       p

P      Pp      Pp

p      pp      pp

From the cross it is clear that 50% of the offspring will have recessive alleles.

Therefore, the probability of short pea plant when a heterozygous parent cross with recessive is 0.5.

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Look at the apparatus shown. It can be used to compare the amount of __________ given out by different fuels. What one word completes this sentence?

Answers

It can be used to compare the amount of energy given out by different fuels.

Thermometer is that apparatus which is used to measure the temperature of the liquid or solution. Temperature is a measure of the kinetic energy of the molecules that is present in a substance. When we record the temperature of something, we are collect information about how fast the molecules are moving in the liquid by heating the solution.

In this experiment, thermometer is dipped into the solution in order to record the amount of energy given out by burning of different fuels so we can conclude that the apparatus is used to measure the amount of energy given out by burning of different fuels.

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the picture shows a tropical rain forest with a large tree canopy that lets through very little sunlight. Which of the following is a form of life that survives well in this environment?

A. fungi that enjoy the dark forest floor and help to get rid of animal and plant waste.

B. mammals that need moist solid of the forest floor in which to lay their eggs.

C. plants that use moonlight to make food and energy for growth.

D. insects that require no water because rainfall is blocked by the canopy

Answers

Answer: AAAAA

Explanation:

Answer: A!

Explanation:

Help me on this please

Answers

Answer:

a is the answer the the following question

PLEASE HELP !! ILL GIVE 40 POINTS ; PLUS BRAINLIEST !! DONT SKIP ANSWER.

Answers

Answer:

The answer is D.

Explanation:

Only 11% of freshwater is in swamps, so option A is incorrect.

The groundwater on earth appears to be a fraction of the freshwater, so option B is incorrect.

We can see that only 3% of the Earth's water is freshwater, so option C is incorrect.

68.7% of Earth's freshwater is found in icecaps and glaciers, meaning it is the majority of Earth's freshwater. Therefore, option D is correct.

The fossil record indicates that in some cases reasonably well-defined species appear suddenly and remain unchanged for a long time before they become extinct. This phenomenon is referred to as

Answers

Answer:

The correct answer is - punctuated equilibrium.

Explanation:

The phenomena represent punctuated equilibrium, according to which the new well-defined species appear suddenly and quickly. These species remain unchanged for a long time as they occur because of mutations in genes.

This phenomenon suggests that once it made in the fossil record the population becomes stable for a long time until they become extinct and have to adapt to the changes take place to survive.

Is there any one who knows how to answer the biology question above

Answers

Answer:

1.. 10,000 joules

2. 1,000 joules

3. 100 joules

Explanation:

This is due to the 10% Rule. The 10% Rule states that on average 90% of energy stays at its current level while 10% is passed down when the holder of the energy is consumed.

Answer:

Tertairy=0.1

secondary=1

primary=10

10 Many organelles in a cell are bound by membranes. Which organelles consist of
membranous tubes and sacs and serve as part of the cell's packaging and transport
system?
F. mitochondria and lysosomes
G. mitochondria and chloroplasts
H. ribosomes and endoplasmic reticulum
I. Golgi apparatus and endoplasmic reticulum

Answers

Answer:

Golgi Apparatus and Enoplasmic Reticulum

Explanation:

Both organelles are membranous organelles that assist in the function of packaging and transport. Endoplasmic reticulum is the site for protein synthesis while the Golgi Apparatus serves as the delivery system.

I hope this helps!

The predicted values for the four phenotypes form a ratio. The simulated values match this predicted ratio.

Answers

Answer:

9:3:3:1 ; approximately

Explanation:

Just did it

Drinking too much water during exercise can cause a condition in which the concentration of sodium in the blood is lower than normal. What immediate response what are the blood cells have in this condition?

Answers

Explanation:

People may also drink excessive water because of a psychiatric disorder called psychogenic polydipsia. The result is too much water and not enough sodium. Thus, overhydration generally results in low sodium levels in the blood (hyponatremia),which can be dangerous.

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An organism's cientific name identifies the ______ and the _____ in which the organism belongs

Answers

Answer:

Genus, Species

Explanation:

Genus and Species, when combined, give you the scientific name of an organism. For example, humans are called h0mo sapiens. Our genus is h0mo and our species is sapiens. Hope this helps :)

[15 POINTS!!]

The diagram below shows a food web.
How would the removal of the rabbit population affect the other populations in the ecosystem?

A. The field mouse population will decrease.

B. The snake population will increase

C. The lizard population will increase.

D. The tree population will decrease

Answers

Answer:

C. because the grasshopper will have less competition to eat Grass letting them reproduce more, since the grasshopper's population increases there will be more food for the lizards helping them reproduce more.

why water is essential for all living things

Answers

Answer: Living organisms need water to survive. Organisms require water to break down food molecules or generate energy during the respiration process. Water also helps many organisms regulate metabolism and dissolves compounds going into or out of the body.

If a color-blind female wants to have children with a normal male, what is the probability one of their sons could be color blind? (Only the sons should be factored into this calculation...) One of their daughters color blind? One of their daughters a carrier? In addition, determine the genotypes of the two parents.​

Answers

Answer:

Mother´s genotype: X⁻ X⁻  Father´s genotype: X⁺ Y Son´s genotype: X⁻ YDaughter´s genotype: X⁺X⁻The probability of having a color-blind son, X⁺Y, is 2/2 = 100% The probability of having a color-blind daughter, X⁻X⁻,  is 0% The probability of having a carrier daughter, X⁺X⁻, is 2/2 = 100%

Explanation:

Due to technical problems, you will find the complete explanation in the attached files

   

Why do specialized plant cells lose their ability to divide

Answers

Answer:

The cells of permanent tissues do not divide. Cells derived from divisition of meristematic to see take up specific role and lose the ability to divide

Explanation:

Answer: In older years, many of the cells experience wear and tear. As a result, they lose their ability to divide or repair themselves.

Explanation: I hope this helps, may God bless you! :)

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A) Complete the sentences with appropriate modal verbs.1. ____________ you ____________ to get up early?2. Theyre having a baby. They ____________ have to buy a bigger house.3. Im sorry I ____________ help you with your homework.4. ____________ we go out tonight?5. Why dont you apply for that job? You ____________ get it.6. I think we ____________ do something to stop pollution.7. ____________ I borrow this book?8. My wife is waiting for me. I ____________ not be late. Find all the real zeros of the function What is the galaxy that our solar system is located in? A chemist needs to determine the concentration of a solution of nitric acid, HNO3. She puts 905 mL of the acid in a flask along with a few drops of indicator. She then slowly adds 0.200 M Ba(OH)2 to the flask until the solution turns pink, indicating the equivalence point of the titration. She notes that 235 mL of Ba(OH)2 was needed to reach the equivalence point.Required:a. How many moles of Ba(OH)2 are present in 225 mL of 0.200 M Ba(OH)2?b. How many moles of HNO3 are present if 4.50 x 10^2 mol of Ba(OH)2 was needed to neutralize the acid solution? (Express your answer numerically in moles) Guys can you please help me. If you guys see this guy and tells you to download stuff and gives you link dont trust them there a hacker his name is( Alan890426 ) Tell whether the difference between two negative integers is always, sometimes, or never positive. The difference between two negative integers is (blank) positive. Question 2 Explain your reasoning. 1) Its always positive because the first integer is always greater. 2) Its positive only if the first integer is greater. 3)Its never positive because the first integer is never greater. HELP QUICK!!!!!!!! ITS DUE TODAY (NO LINKS OR YOU WILL BE REPORTED) have another one that I need done ASAP please and thank u!!! Help me with this work plasr Combine like terms in order to simplify theexpression below.15 - 5y - 5 + 3y A bag cohtains 4 red marbles, 5 blue marbles and 3 green marbles. If two marbles are drawn out of the bag, what is the exact probability that both marbles drawn will be blue? The amount of fluid displaced by an object equals the __________ of the object. Solve equation r/3-4=11 r = 45 r = 35r = 33r = 28 Select the sentence that avoids a comma splice.Option 1: My son graduates from college in a few weeks, he's going into the Navy.Option 2: He's going to Officer Candidate school, he'll be an Ensign in a few months.Option 3: It's all very exciting; it's been a dream of his since he was a young boy.Option 4: Time goes by so quickly, I can't believe he's all grown up. What is the slope of the line? Drag the tiles to write a numerical expression for the following calculation.Divide the difference between 70 and 20 by 25. asap plssssss helpppppppp what are these in spanish 1. the knife:2. the spoon:3. the salt:4. the sugar:5. the napkin: El radio de la base de un cono circular recto mide 18.8 cm. Cul es el mayor valor que se puede tomar la altura h, para que el volumen sea mximo 416.4 centmetros cbicos? 3.The probability of drawing a pearl bead out of a bag of mixed beads is 2/3. What is the probability of drawing a bead which is NOT pearl? What is the purpose of chloroplasts, and why do you think it is missing from the root hair cell?