How to get Live Imaging of the Cellular Genome via CRISPR System?
The spatiotemporal organization of chromatin structure plays a critical role in regulating lineage-specific gene expression during cellular differentiation and embryonic development, via contributing to the functional output of the genome, which can be dynamically amplified or suppressed. However, there is little known about how the genomes being modified and how their structural organization regulating function output.
Answer:
a catalytically inactive Cas9 enzyme (dCas9) is fused to fluorescent labels (e.g., GFP)
Explanation:
The CRISPR-Cas9 genome editing system consists of two parts: 1- a Caspase enzyme called 'Cas9' with catalytic activity that cuts DNA strands by hydrolyzing phosphodiester bonds, and 2-a single guide RNA (sgRNA) that directs Cas9 to target sites with complementarity to nucleotides of the sgRNA in order to edit these sequences. Using a catalytically dead Cas9 (dCas9) it is possible to repurpose the CRISPR-Cas9 system, for example, by fusing dCas9 to epigenetic enzymes for epigenome editing or by fusing dCas9 to the Green Fluorescent Protein (GFP) for imaging of the cellular genome. Thus, it is possible to use dCas9 to create a customizable DNA labeler that can be complemented with a fluorescence microscope and thus track multiple genomic loci in living cells.
Which micro-nutrient is NOT manufactured by living things?
A) Vitamins
B) Carbohydrates
C) Minerals
D) Fats
Answer:
Minerals is the micro-nutrient is not manufactured by living things
Help please! The question is in the image below
Answer: A. The cells produced sugar and oxygen during photosynthesis
Explanation: Plants today make use of this same trait, absorbing the sun's energy and air to create food (sugar) and oxygen (as a by-product)
How many question are on the biology MCAS for 9th grade this year
Answer:
Should be between 50 and 60. I'm assuming about four to six will be open repsonse.
Explanation:
Past experience.
According to Darwin, ______ in species could result from many ______ over long periods of time.
A. population growth; births
B. big changes; small changes
C. mating; interactions
D. xtinction; deaths
According to Darwin, [tex]\sf\purple{big\:changes}[/tex] in species could result from many [tex]\sf\pink{small\:changes}[/tex] over long periods of time.
B. big changes; small changes ✅
[tex]\circ \: \: { \underline{ \boxed{ \sf{ \color{green}{Happy\:learning.}}}}}∘[/tex]
When one glucose molecule travels through the glycolysis processes, four molecules of ATP are generated. Is it accurate to suggest that glycolysis produces two ATP molecules?
Answer:
No
Explanation:
Technically, this is inaccurate. To say that glycolysis produces two ATP molecules strays from the input of ATP needed. Overall, 4 ATP molecules are produced during glycolysis. However, there is an input of 2 ATP molecules at the beginning. Therefore, the correct statement would be that glycolysis produces 2 net ATP molecules, not that glycolysis produces 2 ATP molecules. (The word net makes a big difference!!).
100 POINTS!! Please answer all questions, will report you if you don't give a serious answer
Answer:
1. b
2. l
3. i
4. c
5. k
6. y
Explanation:
state the role of alcohol in this investigation
In guinea pigs, the color of the fur is determined by a single gene with two alleles control. The brown allele (B) is dominant over the white allele (b). Two heterozygous guinea pigs are crossed. Draw a genetic diagram with the genotypic, phenotypic and ratio information of the P₁ and F ₁ generations of this cross.
Answer:
See
Explanation:
Given:
Brown allele (B) is dominant over the white allele (b).
It will be assumed that the alleles are not related to the X and Y chromosomes.
See attached diagram for details of the general case (for both homo-zygous and hetero-zygous parents)
For both parents hetero-zygous,
P1:
Genotype: Bb (100%)
Phenotype: brown (100%)
F1:
Genotypes : BB, Bb, bB and bb in equal proportions, i.e.
BB (25%), Bb (50%), bb (25%)
Phenotypes: 75% brown (from BB and Bb), 25% white (from bb)
Most of the solar radiation absorbed by Earth's surface is later radiated back into space as to which type of electromagnetic radiation?"
Answer:
The sun's visible wavelengths of radiation pass easily through the atmosphere and reach Earth. Approximately 51% of this sunlight is absorbed at Earth's surface by the land, water, and vegetation. Some of this energy is emitted back from the Earth's surface in the form of infrared radiation
Explanation:
In which direction will the object pictured below be accelerating?
Which of the following cells do not have specialized tasks in an organism? 1.) prokaryotic cells 2.) eukaryotic cells 3.) animal cells 4.) plant cells
Answer:
A. Prokaryotic cells
Explanation:
Prokaryotic cells don't have organelles
There are three criteria that scientists use to classify kingdoms. What are they?
Answer: cell type, ability to make food, and number of cells in their body
Explanation:
4. Ask a question about the relationship between two objects
Answer:
Is the two objects depends on each other or not?
Explanation:
If one body depends on the other object it means that there is a direct relationship between these two objects because if increase occur in one object, the value for other object also increases and vice versa while on the other hand, if the two objects independent or does not depend on each other, there is no direct or inverse relationship present among each other that effect one another.
What result allowed Dintzis to conclude that proteins are synthesized from the N terminus to C terminus
Answer:
The more newly synthesized peptides had greater levels of radiolabeled H
Explanation:
Using immature red blood cells (reticulocytes), Dintzis showed that polypeptide chains of hemoglobin were created from N-terminal to the C terminus during translation. Dintzis labeled with radioactive amino acids that were being actively translated, subsequently digested the translated proteins with trypsin and finally determined the amount of tryptic protein fragments. From this experiment, Dintzis observed that the radioactive products were concentrated in the C-terminus (i.e., labeled amino acids appeared first in the C terminal tryptic peptides), thereby showing that the direction of protein synthesis is from the N terminus to the C terminus.
The dissolved waste molecules produced by the cells are removed primarily from the blood by the what?
A. large intestine
B.kidneys
C. skin
D. lungs
What is the answer to number 1
Answer:
TTT GCC AGT
Explanation:
Need help please and thanks
Answer:
The concentration of the oxygen is higher outside of the cell and the oxygen will move inside or towards the cell since it is higher in the outside and lower inside the cell and the transport is passive and it doesn't require energy.
What happens to animals because of plastic trash in the ocean ?
Answer:
They suffocate and choke because of the plastic waste, they also feel trapped in there and sometimes smaller animals get stuck in there
Answer:
Fish, seabirds, sea turtles, and marine mammals can become entangled in or ingest plastic debris, causing suffocation, starvation, and drowning. ... Plastic waste kills up to a million seabirds a year. As with sea turtles, when seabirds ingest plastic, it takes up room in their stomachs, sometimes causing starvation.
Explanation:
A 14-year-old boy develops a pruritic vesicular rash on his face and hands 3 days after hiking in the woods. Which of the following are most likely to be involved in his illness?
a. Antibody, complement C5-9
b. Circulating immune complexes; complement C5a, neutrophils
c. Cytotoxic T lymphocytes
d. IgE, mast cells, histamine, cytokines
e. Lymphocytes, cytokines, macrophages
Answer:
The correct answer is: E) Lymphocytes, cytokines, macrophages.
Explanation:
Given the symptoms and how much time they took to appear, the 14-year-old boy seems to be having delayed-type hypersensitivity (also called Type 4 hypersensitivity), causing a reaction called Contact Dermatitis. Type 4 is the only type of hypersensitivity that does not require the intervening of an antibody to occur as it happens because of hapten-specific T cells that will generate inflammation and the tissue damage that is usually accompanied by it.
Delayed-type hypersensitivity is characterized for happening at least 48 hours after the contact with the antigen, and will involve activated T lymphocytes (specifically CD4+ Th1), which will secrete cytokines (most importantly Interferon Gamma and IL-2) thus attracting macrophages to the tissue to phagocyte the antigen and produce inflammation.
In the picture below the purple circles represent salt molecules. Which way would
the water molecules move across the membrane
Right to left toward the higher concentration of salt
Left to right away from the higher concentration of Salt
Which statement best describes the components of nucleic acids?
Answer:
The statement which describes best about nucleic acids is: They are made up of 'nitrogen bases', 'sugars' and 'phosphates'.
Explanation: Nucleic acids are biomolecules that are known to all forms of life.
what kind of graph is this?
Answer:
Explanation:
financial chart
Which word best describes the response the seedlings are shown?
. The area of particle 1 is
2.5 mm². The area of particle 2
is 0.5 mm². The area of particle
1 is how many times as big as
the area of particle 2?
Answer:
The correct answer is - 5 times
Explanation:
Given:
area of P1 = 2.5
area of P2 = 0.5
Solution:
Let assume:
area of P1 is x times of the area of P2
then the equation would be:
=> 2.5 = x*0.5
=> 2.5/0.5 = x
=> 5 = x
Thus, area of P1 is 5 times to the area of P2
The vertical acis on a graph is called the y-axus for scientific data what usually placed along the y-axis
Answer:
dependent variables
g The lac operon - TRUE or FALSE Before the induction of the lac operon, lactose transporters are already present in the cell membrane. TRUE Lactose initially enters the cell using a transport protein.
Answer:
True
True
Explanation:
Lactose transporter are not absent from the membrane before induction. They are already present in the cell membrane. Lactose requires some transport medium, for this reason protein acts as transport medium for the lactose.
Particulates can be removed from smokeslack emissions by which of the following methods?
_________ is a form of spina bifida in which a saclike cyst filled with CSF protrudes through the spinal defect but does not involve the spinal cord. a. Meningomyelocele b. Meningocele c. Myelomeningocele d. Spina bifida occulta
Answer:
b. Meningocele
Explanation:
Meningocele is a congenital deformity where there is a sac protruding from the spinal column, which is not related to acute neurological conditions. Thus, meningocele is caused by a protrusion of the meninges (i.e., membranes that envelop the brain and spinal cord) and cerebrospinal fluid but without accompanying nervous tissue. About 1,500 babies each year born with this type of spinal birth defect. Moreover, spina bifida occulta is a mild type of spina bifida caused when one or more vertebrae do not fully form during pregnancy. Finally, meningomyelocele (also known as myelomeningocele) is a severe type of spina bifida caused when the spinal cord and nerves develop outside of the body.
Answer:
b. Meningocele
Explanation:
Meningocele is a form of spina bifida in which a saclike cyst filled with CSF protrudes through the spinal defect but does not involve the spinal cord.
Define Vascular tissue?
Answer:
Vascular tissue is a complex conducting tissue found in vascular plants that is made up of several cell types. The xylem and phloem are the two main components of vascular tissue. Internally, these two tissues carry fluid and nutrients.
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