The antibiotic rifampicin blocks transcription in many bacteria. In the presence of the antibiotic, adding sigma factor has no stimulating effect on RNA synthesis. A footprinting experiment on a rifamycin treated sample shows that the polymerase remains located at the promoter site in the presence of eNTPs and Mg2 ion. What step of transcription is rifamycin likely to block

Answers

Answer 1

Answer:

transcription initiation

Explanation:

Transcription is a process by which genetic information from DNA is used to synthesize an RNA molecule, usually, a messenger RNA (mRNA), which is subsequently utilized to synthesize a protein by a process called translation. Transcription in prokaryotes has three steps:

1-Initiation: the RNA polymerase is a multisubunit enzyme (holoenzyme) composed of two α, one β, one β’ and one ω and σ subunits (α2ββ’ωσ). This holoenzyme binds to the promoter region of the template DNA strand.

2-Elongation. The sigma σ factor of the holoenzyme is released and the complex and the core enzyme (α2 ββω) moves along the template strand, thereby producing an mRNA sequence

3-Termination. This step can be Rho-dependent, where a protein named "Rho" recognizes the termination site and stop transcription, and Rho-independent (transcription continues until the termination sequence is reached).

In consequence, Rifamycin is likely to block the initiation of transcription because the core RNA enzyme needs to bind the sigma factor (σ) for initiation of transcription in bacteria.


Related Questions

Look at the food web shown above.

Which organisms get energy from consumers?

Answers

D, all of the above.

Fish in flowing water tend to face:
O Downstream
O No particular direction
Ο Ο Ο
O Perpendicular to the current
Upstream

Answers

Answer: Upstream

Explanation:

3.) How do you know when the first day of the menstrual cycle is? What happens?
4.) What is the function of FSH?

Answers

Answer:

3.) How do you know when the first day of the menstrual cycle is? What happens?

A.) first day of menstrual bleeding is considered Day 1 of the cycle.

4.) What is the function of FSH?

A.) FSH helps control the menstrual cycle and stimulates the growth of eggs in the ovaries.

-TheUnknownScientist

Answer: 3. Day 1 of your cycle is the first day of your period, meaning the first day of full flow (spotting doesn't count). During this time, the uterus sheds its lining from the previous cycle. Between days 1 – 5 of your cycle, new follicles (sacs of fluid containing eggs) begin to develop within your ovaries. 4. FSH plays an important role in sexual development and functioning. In women, FSH helps control the menstrual cycle and stimulates the growth of eggs in the ovaries. FSH levels in women change throughout the menstrual cycle, with the highest levels happening just before an egg is released by the ovary.

Explanation:

Decay product of Potassium-40

Answers

Answer:

calcium-40

Explanation:

reference table

How are the outputs (Carbon dioxide water and energy) of respiration used

Answers

Answer:

During the process of cellular respiration, carbon dioxide is given off as a waste product.

This carbon dioxide can be used by photosynthesizing cells to form new carbohydrates.

Also in the process of cellular respiration, oxygen gas is required to serve as an acceptor of electrons.

Identify all statements that accurately describe the structure of the pyruvate dehydrogenase (PDH) complex. E2 contains three domains. The complex contains multiple copies of each of three enzymes. A regulatory kinase and phosphatase are part of the mammalian PDH complex. The core of the complex is made up of several copies of E1 . Several copies each of E1 and E3 surround E2 .

Answers

Answer: The statements that correctly describe the structure of pyruvate dehydrogenase (PDH) complex are:

--> E2 contains three domains.

--> A regulatory kinase and phosphatase are part of the mammalian PDH complex.

--> Several copies each of E1 and E3 surround E2 .

Explanation:

Enzymes are proteins that speeds up a metabolic reaction by lowering the amount of energy needed to start the reaction. The type of enzyme in a cell will determine the metabolic processes that will occur in it.

Pyruvate dehydrogenase complex which is located at the mitochondria matrix, is a group of enzyme that catalyzes the breakdown of pyruvate, which is the end product of glycolysis, to form acetyl-CoA. The product formed from the reaction which is acetyl-CoA in turn enters the citric acid cycle where it is further oxidized for cellular respiration.

The structure of Pyruvate dehydrogenase complex consists of:

--> Three catalytic enzymes: These include;

• E1: This is the first enzyme of the PDH complex called pyruvate dehydrogenase. It catalyzes the removal of one carbon atom from pyruvate which leads to the production of hydrohyethyl.

• E2: This is the second enzyme of PDH complex called dihydrolipoamide S-acetyltransferase. It catalyzes the transfer of hydroxyethyl group to oxidized form of covalently bound lipoamide, and the resulting acetyl group is then transferred to free coenzyme A to form ACETYL-CoA and reduced dihydrolipoamide. Several copies each of E1 and E3 surround E2 and it consist of three DOMAINS. These domains include: N-terminal 9 kDa lipoyl domain, 4 kDa peripheral subunit-binding domain and C-terminal 28 kDa catalytic (acetyltransferase) domain.

• E3: This is the third and last enzyme of PDH complex called the flavoprotein dihydrolipoamide dehydrogenase. It re-oxidizes the lipoyl group of dihydrolipoamide-E2 to form lipoamide-E2 and NADH.

--> Two regulatory enzymes: The activities of E1 which is the rate limiting step of PDH complex regulated by phosphatases (activators) and kinases (inhibitors).

--> A binding protein: these aids in the binding of E2 to E3 enzyme in the core of PDH complex.

Energy: Mastery Test
2.
Some organisms in an ecosystem obtain energy and nutrients from plants. These organisms store the energy in their bodies. What happens to
the energy in the ecosystem when the organisms die?
ОА.
Some of the energy is used by decomposers, and some of the energy is released into the environment as heat.
OB. A portion of the energy is destroyed, which means the ecosystem must take in more energy from the universe.
Ос.
Most of the energy is converted into nuclear energy by decomposers, and the rest of the energy is absorbed by plants.
All of the energy is lost to the environment, which decreases the total amount of energy available in the ecosystem.
OD
Reset
Next
0

Answers

Answer:

energy used by decomposers

Explanation:

Energy used by decomposers are the ecosystem obtain energy and nutrients from plants.

What is Ecosystem?

An ecosystem, also known as an ecological system, is made up of all the organisms and the physical setting in which they live. The nutrition cycles and energy flows connect these biotic and abiotic elements.

Photosynthesis is how energy enters the system and is absorbed by plant tissue. Animals play a significant part in the transfer of materials and energy through the system by eating plants and one another.

In addition to facilitating nutrient cycling by transforming nutrients held in dead biomass back into a form that can be easily utilised by plants and microorganisms, decomposers also release carbon into the atmosphere through the breakdown of dead organic matter.

Therefore, Energy used by decomposers are the ecosystem obtain energy and nutrients from plants.

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Events that take place in a biome are shown in the diagram below.




What information is represented in the diagram?

A

Respiration and photosynthesis are connected.


B

Transpiration is related to the nitrogen level in bacteria.


C

Predators and their prey are involved in many interactions.


D

Decomposers release a material that is utilized by other organisms.

Answers

Answer:

D - Decomposers release a material that is utilized by other organisms.

Explanation:

The cycle represented is the nitrogen cycle. In it bacteria of decay will break down or decompose plant and animal remains and wastes.

The information represented in the diagram is that decomposers release a material that is utilized by other organisms. So, the correct option is D.

What do you mean by Biome?

A Biome may be defined as a very large area that is characterized by a particular type of vegetation.

The diagram represented here defines the nitrogen cycling in the atmosphere. Decomposers convert the dead and decaying organic matter into inorganic ones which are easily consumable for the plants.

Decomposers directly do not release any material but they convert the material into a specific form which is then utilized by other organisms.

Therefore, the correct option for this question is D.

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Rainforests are habitats for many plants and animals. However, habitat fragmentation is occurring because of human activity. When a rainforest is fragmented, a large area is divided into small patches that are isolated from each other. Species living in these patches are more likely to become extinct as a result of overcrowding and competition.

Which of the following is the best way to minimize the effects of habitat fragmentation on the biodiversity of a rainforest ecosystem?
A.
constructing boundaries and fences around each habitat patch
B.
building natural green pathways across the original habitat to join all the patches together
C.
building concrete roads across the original habitat to join all of the patches together
D.
introducing new species that compete with the existing species in each habitat patch

Answers

Building natural green pathways across the original habitat to join all the patches together. Option B

What is rain forest?

A rainforest is characterized by towering, largely evergreen trees and heavy rainfall. Some rainforests have existed in their current form for at least 70 million years, making them the oldest existing ecosystems on Earth.

Hence we can see that the best way to  minimize the effects of habitat fragmentation on the biodiversity of a rainforest ecosystem is building natural green pathways across the original habitat to join all the patches together.

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Why fission, regeneration and fragmentation, all are considered as methods of asexual reproduction ?​

Answers

Fission (binary and multiple), regeneration and fragmentation are considered as methods of asexual reproduction because all of them involve only one parent and gametes are not formed in them. New individuals produced after cell division are always genetically identical or clone to their parents.

article about guimaras oil spill discussing the effect of hazards and how do people survive in this kind of situation.​

Answers

Answer:

It harms marine birds and animals.

Explanation:

Oil spills are harmful to marine birds and mammals as well as fish and shellfish because this oil makes their movement difficult as well as suffocation problem. People can survive in this kind of situation by removing the oil as much as possible. The oil can't mix with the water due to difference of nature so it can be easily removed from the water. This oil spell affect the environment as well as financial condition of people who are related fish industry because fish population negatively affected.

Which of the following is NOT an example of a pre-zygotic barrier?

behavioral isolation

hybrid sterility

habitat isolation,

mechanical isolation

Answers

Answer:

isolating mechanisms The reproductive characteristics which prevent species from fusing. Individuals mate in their preferred habitat, and therefore do not meet individuals of other Genomic incompatibility, hybrid inevitability or sterility. show that there is little behavioural, mechanical, or post-mating isolation

Explanation:

i hope all help full

The largest number of individuals of one species that an environment can support

Answers

Answer:

I believe you are looking for  the Carrying Capacity.

Explanation:

Where does pollination take place for an orange tree?
A. Flowers
B. Leaves
C. Roots
D. Trunk

( please help due right now )

Answers

Answer: A.
Insects fly to the flower for pollen and nectar

Answer:

its A :)

Explanation:

i got the answer correct on my work

Meiosis is a type of cell division that is specific to

Answers

Meiosis is a type of cell division that is specific to germ cells (gamete cells).

What is Meiosis?

Meiosis may be defined as a type of cell division in which each parental cell is divided into four daughter cells. Each daughter cells are genetically unique to the parental cell and are haploid in nature.

Gamete cells are typically divided by this mechanism of cell division. This is because when toe distinctive gamete cells from male and female are fused together, the resultant zygote will have the entire set of genetic material.

The gamete cells significantly have half the amount of genetic content. Due to this, it is also known as reductional division.

Therefore, meiosis is a type of cell division that is specific to germ cells (gamete cells).

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40 Even a tiny amount of moisture can support the life of mosses, which are nonvascular
plants. Why are mosses good pioneer species?
F. They grow very slowly.
G. They can survive in wet areas.
it. They can create a layer of soil on bare rock over time.
I. They can survive in areas that receive low levels of sunlight.

Answers

Both it.) And I.) Can be answers. You could argue either one is right.

i need help with my biology hw i will try to add a image

Answers

Explanation:

First,click the pic and when you start for your question click on the option for adding image.

Answer:

so, where is the questions........

A group of researchers has made the claim that one particular butterfly species is the sole pollinator of a tree species and that consequently, the decline of the butterfly population will endanger the survival of the tree species. Data were collected and are summarized below. Which of the following data could best be used to either justify or refute the claim the researchers have made about the interdependence of the butterfly species and the trees?

Tree flower size Number of butterfly visits per flower per day Nectar energy per pollination visit (joules) Fruits per flower
Small 1 10 0.88
Medium 9 12 0.79
Large 55 13 0.85

a. The data comparing the fruits produced per flower with the number of butterfly visits are most useful in refuting the claim, because they show that the tree produces fruit even if the butterfly does not visit the flowers.
b. The data collected for large flowers are most useful in justifying the claim, because they show that a large number of butterflies visit flowers of this tree species, obtain energy from nectar, and cause fruit production.
c. The data comparing the number of butterfly visits with the size of the tree flowers are most useful in refuting the claim, because they show that the butterfly does not visit all types of tree flowers.
d. The data showing the nectar energy available for all sizes of flowers are most useful in justifying the claim, because they show that butterflies benefit from the relationship with the tree by obtaining energy from tree nectar.

Answers

Answer:

The correct answer is - A. The data comparing the fruits produced per flower with the number of butterfly visits are most useful in refuting the claim, because they show that the tree produces fruit even if the butterfly does not visit the flowers.

Explanation:

In this experiment, the table shows that specific butterflies did not go to small flower trees or go once a day where is in medium and large flower trees respectively they visit more than small flowers.

Even after not visiting small flower trees the nectar energy and the fruit production is almost similar to the other two trees. This case can occur only in one case if there are one or more pollinators present in this scenario other than this type of specific butterflies.

Thus, the correct answer is - option A.

Which of these is a parasite, A) Venus Fly trap B) Corpse flower C) Corpse Lily D) Grass

Answers

Answer:

Corspe Flower

Explanation:

option b.

I hope this helps

Answer:

c) corpse lily

is not sleeping bad for u
hi I didnt sleep for 16 hours and I have messed up sleep pattern
will i commit no alive

Answers

No many people stay awake for 16 hours or more the only time you should be concerned is if you younger than the age of 13 try to sleep if you can try to get atleast 4 hours if any at all

Answer:

It depends. 16 hours is a long time not to sleep. You need to sleep though. Your body needs at least 8 hours of sleep. But you need at least 5 to 6 hours of sleep. So your body can regenerate itself.

Explanation:

how many toes do velociraptors have

Answers

Answer:

Two toes

Explanation:

What caused the human population to grow so quickly so fast?
the Industrial Revolution
O discovery of fire
the end of World War II

Answers

because the level of chlidren born per day stay at the high level it increases day per day

Which of the following is an example of genetic discrimination?

Answers

Where’s the examples..

Name 1 body system (NOT the digestive
system) that your "fave" food can
impact.
How does your "fave" food impact the
body system you selected?
(Hint: These are the body systems we will
be learning about- Excretory, Nervous,
Muscular, Circulatory & Respiratory. You
can also include Integumentary & Skeletal

My favorite food is Buffalo Wings

Answers

Answer:

I'll use your favorite food.

Explanation:

Buffalo wings have protein since they have meat. Protein helps the body build muscle, so it impacts the muscular system.

PLEASE HURRY I WILL GIVE BRAILIEST TO WHO EVER ANSWERS AND IS CORRECT
Select all benefits of using a wiki for business.


inexpensive

accessible twenty-four hours per day and seven days per week

online conversation

quick and easy editing

document sharing

Answers

Answer:

All of the above

Explanation:

Answer:

Inexpensive, accessible twenty four hours per day and seven days per week, and document sharing

Explanation:

I got it right on the quiz.

If a plant cell and its surroundings are isotonic)
there is no net movement of water into the cell:
the cell becomes

Answers

Answer:

Flaccid

Explanation:

The cell wall provides no advantages when a plant cell is immersed in a hypertonic solution. As the plant cell loses water, its volume shrinks.

A balloon holds 50.0 mL of nitrogen at 25° C and a pressure of 736 mm Hg. According to Boyle's Law, answer the following questions for 2 points each: What will be the effect on the pressure inside the balloon if the volume is decreased to 25 mL? Explain how this would demonstrate Boyle's Law. Explain Boyle's Law in terms of the kinetic energy of molecule

Answers

Answer:

The correct answer is -  would be double which is 1472 mm Hg.

Explanation:

According to Boyle's law that says the relationship between volume and pressure of a gas at constant pressure shows an inverse relationship which means if volume will increase then pressure will be decrease and vice versa.

P1V1 = P2V2 .........Boyle's law

Given: P1 = 736 mm Hg

          V1 = 50 mL

P2 = ?

V2 = 25 mL

Solution:

from the Boyle's law

(P1V1)/V2 = P2

736×50/25 = P2

P2 = 1472

So the decreasing the volume to half increases the pressure double. It proves it follows Boyle's law

PLEASE HELP !! ILL GIVE 40 POINTS ; PLUS BRAINLIEST !! DONT SKIP ANSWER.

Answers

Answer: B archea

Explanation

Answer:

b archean

i think good luck

how many human blood types are there? summarize how blood type is inherited

Answers

Explanation:

There are 4 main blood groups (types of blood) – A, B, AB and O. Your blood group is determined by the genes you inherit from your parents. Each group can be either RhD positive or RhD negative, which means in total there are 8 blood groups.

The diagram below represents some stages in the life cycle of humans. The numbers in the diagram represent various processes in the cycle.




Identify the process(es) represented by 1 and 2. State how these processes (1 and 2) affect the amount of genetic information provided by a parent to its offspring.

Answers

Answer:

The diagram below represents some stages in the life cycle of humans. The numbers in the diagram represent various processes in the cycle.

1. "The processes reduce the amount of genetic information from each parent by half."

During sexual reproduction, a specialized cell division process called meiosis occurs. Meiosis involves two consecutive divisions, resulting in the production of four haploid cells (gametes) from a diploid cell.

2. "Each parent only contributes half of the genetic information that is contained in his or her own cells."

As mentioned earlier, meiosis results in the formation of haploid gametes. Each gamete contains only one set of chromosomes, which is half the number found in the parent's diploid cells.

During meiosis, homologous chromosomes pair up, and genetic recombination occurs between them, shuffling and exchanging genetic information.

3. "Each egg (or each sperm) will carry only half of the genetic information contained in the parent's body cells."

This statement reiterates that gametes (eggs or sperm) are haploid cells containing only one set of chromosomes. When a cell undergoes meiosis to produce gametes, it divides twice, resulting in four cells with half the genetic information of the parent's body cells.

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Which choice represents the simplified exponential expression? (11^-2)^7PLEASE HELP!!! What happens AFTER the battle between Odysseus and the Cicones? Select all of the following substances that can be addicting. Samantha has 52 jelly beans. She decides that she will eat 4 jelly beans per minute, and she wants to create an expression that represents how many jelly beans she has left after x minutes. Which of the following expressions correctly models this situation?Select one:A. 4x + 52B. x + 52C. -x + 52D. -4x + 52~and also if whoever answers doesn't mind, please put an actual explanation since I just wanna be able to fully understand the concept! It would be greatly appreciated! ~ Giving brainliest to the best answer :) Write the ratio 1.5:9 in simplest form. HELPPPP PLSSSSPLSSS PLS I NEED HELL Jewish people were removed from their homeland to other places;African were removed from their homeland through slavery. What is theword for this forced move 1. This question has two parts. First, answer Part A. Then, answer Part B. Part A Which Words describe Ling? Circle all that apply. A. conscientious B. cowardly C. deceitful D. courageous E. boastful F. honestPart BWhich excerpts from the story BEST support your answers to Part A? Choose ALL that apply.A. Standing at the emperor's site, a poor young man named Ling held his hand out to receive the first seed.B. Ling planted the seed deep in the fertole Earth and watered it everyday but after 3 weeks of constant care he saw no sign of life.C. Trembling, Ling walked to the front of the room and bowed to the emperor.D. Ling was certain he was dreaming.E. "Ling was the only one with the courage to be honest, and therefore he is the new emperor!" GIVING 43 POINTS AND BRAINLIEST FOR THE FIRST PERSON THAT ANSWERS AND IS CORRECT!!Translate the following expression9 more than a numberA. 9rB. 9 + wC. 9kD. 9x 4) Graph f(x) 2(3)*. Compare the graph to thegraph of the parent function. Describe the domainand range of f. Please help me with this ASAP ASAP please please help 7.17 x 10' and 8.17 x 1067 Respond if you have seen ateez song fireworks,I'm the one. 6. What did Hitler do to the blonde journalist man who wrote articles against Hitler?Need some help Anthony starts with $400 in his bank account. Over the course of a week, he withdraws $50, deposits $70, and withdraws $20. How much money is in his account? show your work, please Maria had 10.5 pounds of flour. She used all of the flour to fill bags that hold 0.75 pound each. How many bags was she able to fill? (: Find the area of the given shape? Lipids provide much of the dietary energy in the bodies of infants and young children. There is a growing interest in the quality of the dietary lipid supply during infancy as a major determinant of growth, visual and neural development, and long-term health. An article reported the following data on total polyunsaturated fats (%) for infants who were randomized to four different feeding regimens: breast milk, corn-oil-based formula, soy-oil-based formula, or soy-and-marine-oil-based formula. (Use i= 1, 2, 3, and 4 respectively.)Sample Regimen Sample Size Sample Mean Sample SDBreast milk 15 42.9 1.3CO 17 43.1 1.5SO 8 43.7 1.2SMO 14 43.9 1.2What assumptions must be made about the four total polyunsaturated fat distributions before carrying out a single-factor ANOVA to decide whether there are any differences in true average fat content?a. The distribution of polyunsaturated fat percentages for each of the four regimens must be uniform with equal variances.b. The distribution of polyunsaturated fat percentages for each of the four regimens must be normal with unequal variances. c. The distribution of polyunsaturated fat percentages for each of the four regimens must be normal with equal variances.d. The distribution of polyunsaturated fat percentages for each of the four regimens must be binomial with unequal variances. The height of a cylinder is twice the radius of its base