What is the restriction site recognized by EcoRI?

Answers

Answer 1

Answer:

In biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G/AATTC, which has a palindromic, complementary sequence of CTTAA/G.

Explanation:

Hopefully that helps, I had previously learned about this stuff too, super tough stuff!

Answer 2

Answer:

G|AATTC

CTTAA|G

Explanation:

EcoRI is a bacterial restriction enzyme that cuts DNA into fragments at defined sites. These specific sites are denoted by a specific sequence:

G|AATTC

CTTAA|G

The | symbol shows the site of the cut.

These enzymes are used to create cuts in DNA to enable cloning in genetic engineering.


Related Questions

Which of the following is NOT an example of skill-related fitness? A. reaction time B. balance C. flexibility D. coordination

Answers

Answer:

C. Flexibility

Explanation:

There are 6 skill related fitness components: agility, balance, coordination, speed, power and reaction time.

Answer:

Flexibility

Explanation:

please helpp

The strength of one’s ethnic identity can increase or decrease as a result of stereotypes or acts of discrimination within a society.
t or f

Answers

Answer: T

Explanation: people could see one differently because of their ethnicity as a cause of stereotypes or discrimination

Answer:

true

Explanation:

Which of the following explains why fruit snacks with the newest Disney movie characters on them are only on the shelves for six
months?
ANSWER A the movie is no longer playing in theatres

Answers

I need more options I don’t understand the question.

In determining whether there is conductive
hearing loss, it is important to perform
A) a Speech-in-Noise test
B) a test to determine which frequency is heard
at different decibels
C) a Rinne test
D) all of the above.

Answers

Answer: My answer would be C but wait until some one else replys i dont wanna mess you up.

Explanation:

what are the four elements of fitness programs?

Answers

Answer:

aerobic fitness,muscular fitness,flexibility,stability and balance

Explanation:

In regard to exercise, which of the following statements about safety consideration is correct?
A.
In hot weather, exercising between 10 a.m. and 4 p.m. is best.
B.
Exercising at 100% of your maximum heart rate is best.
C.
Exercising one hour before eating is best.
D.
Remaining well-hydrated while exercising is best.


Please select the best answer from the choices provided.

A
B
C
D

Answers

Answer:

d is the answer because if you stay hydrated you will have the cost of dyingn

Explanation:

Answer:

The answer is D.

Explanation:

how can I get a go
plz help need some advice ​

Answers

loosiento hay corona envotella tienes q casarte con tu madre denada por todo quieres un donete?

Advice for what?!!!!

ture or false The fallopian tubes are a bilateral structure of the female reproductive system.

Answers

True is the correct answer

The statement " The fallopian tubes are a bilateral structure of the female reproductive system" is definitely true.

What is a Fallopian tube?

A fallopian tube may be defined as a reproductive part of the mammalian female that carries the egg from the ovary to the uterus. This part provides the route for fertilization.

The structure of the fallopian tube is bilateral which means the procedure is done by both tubes significantly. There are two tubes, one on each of the uterus and meets the region of the ovary.

Therefore, the statement " The fallopian tubes are a bilateral structure of the female reproductive system" is definitely true.

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is tomato a fruit or a vegatable??

Answers

Answer:

fruit

Explanation:

Answer:

Tomato is actually a fruit

Which item belongs to one of the five food groups in the USDA's MyPlate Food Guidance System?
A.
oatmeal cookie
B.
milk
C.
olive oil
D.
salt

Answers

Answer:

B. Milk

Explanation:

Milk is a protein which is on the myplate.

Some injuries can only be corrected surgically.

Answers

Is this supposed to be a true/false question? If so, the answer is true.
Answer: True.


Explanation: This is especially true when the injuries are life-threatening or it could lead to a loss of a limb. It needs immediate medical attention. Bullet wounds or stab wounds to the vital organs require surgery that could save a person’s life.

what message is presented about being a man or a woman​

Answers

Answer:

The message presented is that man and woman are equal in their role in competing the challenge. In the Nike commercial "men and women challenge", they are both equally competitive to finish the line.

Explanation:

Does the abdominal support the vertebrae?

Answers

Answer:

yes i think

Explanation:

What is the importance of undergoing health appraisal?

A. To control the risk of developing certain medical conditions

B. To detect your present medical problems

C. To identify the illness you are experiencing

D. To save your life from illness​

Answers

The answer is letter a
A. To control the risk of developing certain medical conditions

Why is it difficult to discuss sex-related topics?

Answers

Answer:

because this is a school sight for homework

Explanation:

1.The risk of obesity, heart disease and cancer is increased by a diet that is :
A- trans fat
B- saturated fat
C- cholesterol
D- all of the above

2. Essential amino acids are:
A- vitamins that dissolve in fat
B- the nine amino acids the body can’t produce
C- come from greens, legumes, nuts and seeds
D- are units of energy produced by proteins

Answers

1. Is “all of the above” and I don’t know about the 2. one

The risk of obesity, heart disease and cancer is increased by a diet that is encompassing trans fat, saturated fat and cholesterol. The essential amino acids are the nine amino acids the body can’t produce. The correct options are D and B respectively.

What are amino acids?

Amino acids are compounds that form proteins when they combine. The fundamental components of life are amino acids and proteins.

Amino acids are left over after proteins are digested or broken down. To help the body, the human body uses amino acids to make proteins: Food should be broken down.

Essential amino acids are those that must be obtained through diet because the body cannot produce them.

High salt, sugar, saturated fat, and refined carbohydrate consumption raises your risk of having a heart attack or stroke over time.

If you're concerned about your heart, you should avoid these on a regular basis. However, rather than focusing on a single bad food, it is better to concentrate on your overall diet.

Thus, D and B are the correct options respectively.

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Brenda is married with 1 child. She has frequent blackouts with loss of memory. She occasionally finds herself in strange locations, not knowing how she got there. Her husband claims that she is sometimes quiet and sometimes boisterous with distinct changes in her voice and mannerisms. Her neighbor reports that sometimes she leaves in very flashy clothing, and other times, she has appeared to be intoxicated. Brenda is suffering from:
Question 2 options:


Dissociative Identity Disorder


Schizophrenia


Dissociative Amnesia


Personality Disorder

Answers

the answer is D.I.D. aka dissociative identity disorder.

In the disorder called "dissociative identity disorder," the "alters," or distinct personalities:
O a. may all be present simultaneously.
O b. are typically all the same age.
O c. are typically all the same gender.
O d. can be of different ages and sexes.

Answers

D. Can be of different ages and sexes.

A dissociative condition can affect people of diverse ages and sexes, hence option D is correct.

What is dissociative identity disorder?

The person's alternative selves are their "Alters." Some DID sufferers have as many as 100 alters. Alters frequently differ greatly from one another.

Disconnections between ideas, identity, awareness, and memory are hallmarks of dissociative disorders, which are defined by an unconscious attempt to escape reality.

Depending on the type of dissociative disorder you have, you may experience the following signs and symptoms. Amnesia is the loss of memory for specific times, events, persons, and private information.

Therefore, the identities may differ in terms of gender, ethnicity, hobbies, and interactions with their surroundings, hence option D is correct.

Learn more about the disorder, here:

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HELP plzz
The strength of one’s ethnic identity can increase or decrease as a result of stereotypes or acts of discrimination within a society.


Please select the best answer from the choices provided

T
F

Answers

Answer:

True

Explanation:

Hope this helps!

Answer: trueeeeeee

Explanation:

Fruit can be cooked in all of the following ways EXCEPT

baking
boiling.
broiling.
poaching.

Answers

Answer:

the answer is poaching

Answer:

its boiling

Explanation:

When census numbers show changes in a state’s total population, what is likely to be affected in the state?

Answers

Funding and representatives in the house

The two main cavities of the body are the chest (thorax) and abdomen.
Question 1
True
False
Question 2
The liver and stomach are in the abdomen.
True
False
Question 4
Which of the following is NOT located in the abdomen?

A)Lungs

B)Liver

C)Intestines

D)Kidneys
Question 5
The heart is in the abdomen.
True
False

Answers

Question 1: true
Question 2: true
Question 4: a) lungs
Question 5: false

Identify three effective, nonviolent methods for resolving conflict.
A. Respond as though you are threatened.
B. Stay in control of your feelings and emotions.
O C. Talk to a trusted adult.
D. Stop and think before you act.

Answers

Answer: B, C, D     A.PEX Confirmed 100%

↓↓ Explanation: ↓↓

The reason is that you have to control your feelings and emotions so others don't see your emotions and feelings.

Talk to a trusted adult to talk out your problems and how you feel.

Stop and think before you act if you don't stop and think before you act you could mess things up or say something you didn't want to say that you will regret saying.

hope this helps :-)

please mark me as brainlyest

Three effective, nonviolent methods for resolving conflict are: staying in control of feelings and emotions; talking to a trusted adult; and stopping and thinking before acting. These are B, C, and D.

What is conflict?

Conflict is a natural and inevitable part of human interaction. However, conflicts can become harmful and destructive when they are not managed effectively. In order to resolve conflicts in a nonviolent and constructive way, it is important to use effective methods that can de-escalate tensions and promote mutual understanding and respect. Staying in control of emotions is an important method for resolving conflict because emotions can often fuel the conflict and make it more difficult to find a solution.

Hence, Three effective, nonviolent methods for resolving conflict are: staying in control of feelings and emotions; talking to a trusted adult; and stopping and thinking before acting. These are B, C, and D.

Learn more about the conflict here.

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An insect is flying towards your eye and you quickly blink. What type of specialized reflex will occur?

Answers

Answer:

u would probably swat it blink say something and move in some way all at the same time

Explanation:

Corneal reflex
Your eye will react in a manner called Corneal reflex

What strategies can a person practice to prevent the use of harmful substances? Avoid activities with others. Confide in a proven trusted adult. Use social media to get peer advice. Seek friendships only with older people.

Answers

Answer:

the correct answer is 'Confide in a proven trust adult'

Explanation:

You cant avoid social interactions.

Social media is the worst place for advice and you dont need to be friends with older folks only.

Answer:

b

Explanation:

Argue in support of food nutrition labeling regulated by the FDA and explain its significance.

Answers

Answer: I got this same question and I was looking for the answers and could not found it but this I what I put. Food nutrition labeling by the FDA is important because it gives customers the ability to view the ingredients listed and find what they are looking for. For example women need more calcium to prevent brittleness in bones.

Answer: A correct answer will indicate that the FDA is responsible for ensuring that food in the United States is safe, wholesome, and properly labeled. These labels include calories, serving size, percent daily value, total fat, cholesterol, carbohydrates, proteins, and vitamins. All values help consumers make better health decisions because they can track caloric intake and fat, as well as the benefits certain foods offer. Consumers can also see what the serving size is and how many servings are in each container, so they can better track what they are putting into their bodies. The answer could also indicate that prior to the existence of the FDA, unregulated medicine shows traveled across the country, with no oversight over products ingredients or quality, or proof of claims on the labels, resulting in great risk of harm to the public.

Explanation:(that is exactly the right answer)

The muscular wall of the uterus is the endometrium ?

True
False

Answers

Answer:

true

Explanation:

The answer is True I know

6. Which is a characteristic of a reliable weight loss plan?
O It suggests getting a physician's input before starting.
It reduces calories below 1,000 calories per day.
ebo
It encourages dieters to avoid the foods that friends and family eat.
It requires the purchase of special supplements or equipment.

Answers

Answer:

It suggests getting a physician's input before starting.

A characteristic of a reliable weight loss plan is It suggests getting a physician's input before starting. The correct option is option (A).

A reliable weight loss plan encourages seeking a physician's input before starting to ensure that the approach is safe and suitable for an individual's specific health needs and goals.

This professional guidance can help address any underlying medical conditions, assess potential risks, and tailor the plan accordingly.

Therefore, A characteristic of a reliable weight loss plan is It suggests getting a physician's input before starting. The correct option is option (A).

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Some of the accessory structures of the female are:


a. ovaries and uterus
b. none of the above
c. fallopian tubes and uterus
d. urethra and vulva

Answers

I'm not sure but I think d

and what's with this question.....

D i think it’s d because that’s what I have to be asked to

what is the definition of each from kingdom to species​

Answers

Wait the definition of what I can answer but this question is confusing.
can u explain a little more i’m confused on what your trying to say
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